The use of CdTe quantum dot fluorescent microspheres in fluoro-immunoassays and a microfluidic chip system. |
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Authors: | Qiang Ma Xinyan Wang Yabing Li Xingguang Su Qinhan Jin |
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Affiliation: | Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, People's Republic of China. |
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Abstract: | Polystyrene fluorescent microspheres prepared by deposition of CdTe quantum dots (QDs) are used in an immunoassay in this study. CdTe QDs/polyelectrolyte multilayers on the surface of polystyrene microspheres have been formed by layer-by-layer self-assembly via electrostatic interactions. As a model antigen, rabbit IgG has been bound to the outermost layer of the fluorescent microspheres. The immunoreaction between fluorescent microspheres/rabbit IgG and the corresponding antibody was confirmed by change of the fluorescence spectrum and competitive immunoassay. This approach allowed detection of the antigen (rabbit IgG) in the range 1-500 mg/L, based on the change in the fluorescence intensity of the reporter (fluorescent microspheres/rabbit IgG). A novel microfluidic chip device with a laser-induced fluorescence system was established and used for the detection of fluorescent microspheres in this study. |
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Keywords: | quantum dots fluorescent microspheres microfluidic chip immunoassay |
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