| 肝细胞生长因子对四氯化碳损伤原代培养大鼠肝细胞的保护作用 |
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| 引用本文： | 邹原,宫德正,孙丽娟,梅懋华. 肝细胞生长因子对四氯化碳损伤原代培养大鼠肝细胞的保护作用[J]. 中国应用生理学杂志, 1997, 0(3) |
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| 作者姓名： | 邹原 宫德正 孙丽娟 梅懋华 |
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| 作者单位： | 大连医科大学生理教研室 |
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| 摘 要： | 本工作采用无血清原代培养大鼠肝细胞法，观察了重组人肝细胞生长因子（ｒ－ｈＨＧＦ）对四氯化碳（ＣＣｌ４）致大鼠肝细胞损伤的保护作用。结果表明，ｒ－ｈＨＧＦ对ＣＣｌ４染毒肝细胞有明显的保护作用。ｒ－ｈＨＧＦ保护组较ＣＣｌ４染毒组细胞存活率显著升高，细胞内丙氨酸转氨酶、钾离子漏出明显降低。结果提示，ｒ－ｈＨＧＦ可减轻ＣＣｌ４对肝细胞膜的损伤，提高细胞膜的结构完整性
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| 关 键 词： | 肝细胞生长因子 丙氨酸转氨酶 钾离子 四氯化碳 肝细胞原代培养 |
PROTECTION OF HEPATOCYTE GROWTH FACTOR AGAINST CARBON TETRACHLORIDE INJURY IN PRIMARY RAT HEPATOCYTE CULTURE |
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| Zou Yuan,Gong Dezheng,Sun Lijuan ,Mei Maohua. PROTECTION OF HEPATOCYTE GROWTH FACTOR AGAINST CARBON TETRACHLORIDE INJURY IN PRIMARY RAT HEPATOCYTE CULTURE[J]. Chinese journal of applied physiology, 1997, 0(3) |
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| Authors: | Zou Yuan Gong Dezheng Sun Lijuan Mei Maohua |
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| Affiliation: | Zou Yuan,Gong Dezheng,Sun Lijuan 1,Mei Maohua |
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| Abstract: | The protective effect of recombinant human hepatocyte growth factor (r hHGF) against carbon tetrachloride (CCl 4) injury in cultured rat hepatocytes was investigated. It was found that r hHGF had cytoprotective effect on hepatocytes anainst CCl 4 poisoning. Pretreatment with r hHGF significantly increased the viability of cultured hepatocytes, reduced the leakage of intracellular alanine transaminase and potassium into the culture medium, as compared with the CCl 4 group. The results suggest that r hHGF may protect hepatocytes against CCl 4 injury through lessening the severity of membrane lesion,preventing intracellular alanine transaminase and potassium leakage and sustaining the integrity of hepatocyte membrane. |
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| Keywords: | recombinant human hepatocyte growth factor (r hHGF) alanine transaminase (ALT) potassiumions carbon tetrachloride (CCl 4) primary hepatocyte culture |
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