Activation of p42 Mitogen-Activated Protein Kinase by Glutamate in Cultured Radial Glia |
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Authors: | López-Colomé Ana María Ortega Arturo |
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Affiliation: | (1) Departamento de Neurociencias, Instituto de Fisiología Celular-UNAM, Mexico;(2) Departamento de Genética y Biología Molecular, CINVESTAV-IPN, Apartado Postal 14 740, México DF, 0700, México |
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Abstract: | The effect of L-glutamate (Glu) and its structural analogs N-methyl-D-aspartate (NMDA), kainate (KA) and -amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA), on the activation of p42 mitogen activated protein kinase (MAPK) was examined in cultured chick radial glia cells, namely retinal Müller cells and cerebellar Bergmann cells. Glu, NMDA, AMPA and KA evoked a dose and time dependent increase in MAPK activity. AMPA and KA responses were blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) whereas NMDA responses were sensitive to 3-[(RS)-2-carboxypiperazin-4-yl)]-propyl-1-phosphonate (CPP) indicating that the increase in MAPK activity is mediated by AMPA/low affinity KA and NMDA subtypes of Glu receptors. The present findings open the possibility of a MAPK cascade involvement in the regulation of Glu-induced gene expression in radial glia. |
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Keywords: | Radial glia glutamate receptors mitogen-activated protein kinases signal transduction |
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