| 纳豆激酶的分离纯化及生化研究 |
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| 引用本文： | 丁贵平,蔡正森,王正刚. 纳豆激酶的分离纯化及生化研究[J]. 氨基酸和生物资源, 2001, 23(3): 13-16 |
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| 作者姓名： | 丁贵平 蔡正森 王正刚 |
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| 作者单位： | 江苏省微生物研究所, |
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| 摘 要： | 用固体发酵工艺制得的纳豆中含有大量溶栓活性成分———纳豆激酶。经过生理盐水浸提 ,(NH4 ) 2 SO4 分级沉淀 ,Sephadex柱层析等步骤 ,可以得一层析纯的纳豆激酶。冷冻干燥品经SDS—PAGE电泳显示有二组分存在
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| 关 键 词： | 纳豆激酶 纳豆 分离纯化 |
| 文章编号： | 1006-8376(2001)03-0013-04 |
| 修稿时间： | 2000-11-17 |
Purificaton and Characterization of Nattokinase |
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| DING Gui-ping,CAI Zheng-sen,WANG Zheng-gang. Purificaton and Characterization of Nattokinase[J]. Amino Acids & Biotic Resources, 2001, 23(3): 13-16 |
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| Authors: | DING Gui-ping CAI Zheng-sen WANG Zheng-gang |
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| Abstract: | The natto made by solid fermentation contains a large amount of thrombuslytic active component-nattokinase. The enzyme was purified to a chromatographic purity with a series of purification procedures including saline extraction, salt-out with ammonium sulfate sephedex gel chromatography and freeze drying. The purified enzyme contained two stainable subunits on the gel of SDS-PAGE. |
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| Keywords: | Nattokinase natto purification |
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