Interleukin 8-inhibitor and inducer of histamine and leukotriene release in human basophils. |
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Authors: | S C Bischoff M Baggiolini A L de Weck C A Dahinden |
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Institution: | Institute of Clinical Immunology, Inselspital, Bern, Switzerland. |
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Abstract: | We have shown previously that interleukin 8 (IL-8) induces histamine and leukotriene release in human basophils exposed to interleukin 3 (IL-3). We now found that pretreatment with low concentrations of IL-8 selectively inhibits this response. Inhibition was significant at 0.01 nM IL-8 and virtually complete at 1 nM, which is about 100-fold lower than the concentration required for induction of mediator release. IL-8 dependent responses were also inhibited, albeit to a lesser extent, by preincubation with neutrophil-activating peptide 2 (NAP-2), but not with connective tissue-activating peptide III (CTAP-III) or platelet factor 4 (PF4). Release induced by C5a, fMet-Leu-Phe, or anti-IgE antibody, by contrast, was not affected. |
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