Purification and characterization of human RNPS1: a general activator of pre-mRNA splicing. |
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| Authors: | A Mayeda J Badolato R Kobayashi M Q Zhang E M Gardiner A R Krainer |
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| Affiliation: | Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL 33136, USA. amayeda@molbio.med.miami.edu |
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| Abstract: | Biochemical purification of a pre-mRNA splicing activity from HeLa cells that stimulates distal alternative 3' splice sites in a concentration-dependent manner resulted in the identification of RNPS1, a novel general activator of pre-mRNA splicing. RNPS1 cDNAs, encoding a putative nucleic-acid-binding protein of unknown function, were previously identified in mouse and human. RNPS1 is conserved in metazoans and has an RNA-recognition motif preceded by an extensive serine-rich domain. Recombinant human RNPS1 expressed in baculovirus functionally synergizes with SR proteins and strongly activates splicing of both constitutively and alternatively spliced pre-mRNAs. We conclude that RNPS1 is not only a potential regulator of alternative splicing but may also play a more fundamental role as a general activator of pre-mRNA splicing. |
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