Comparative study of cellulases and hemicellulases from four fungi: mesophiles Trichoderma reesei and Penicillium sp. and thermophiles Thielavia terrestris and Sporotrichum cellulophilum |
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Authors: | Henri Durand Philippe Soucaille Gérard Tiraby |
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Institution: | Laboratoire de Recherche CAYLA, 99 Chemin de la Salade Ponsan, 31400 Toulouse, France;Centre de Recherche de Biochimie et de Génétique Cellulaires, Université Paul Sabatier, 31062 Toulouse Cédex, France |
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Abstract: | The enzymes produced by two thermophilic fungi claimed to produce heat-stable cellulases see 1,4-(1,3;1,4)-β-d-glucan 4-glucanohydrolase, EC 3.2.1.4] have been compared to those of two mesophilic fungi on the basis of the following criteria: polysaccharolytic spectrum, heat and pH effects on stability and on activity of the different enzymes, and the ability to hydrolyse raw natural substrates. The cellulases produced by one of the thermophiles, Sporotrichum cellulophilum, appeared to be as heat-labile as those from the mesophile Trichoderma reesei; moreover, the former enzyme preparation is the least efficient of the four tested. Thielavia terrestris enzymes are the most thermostable; on the basis of the other properties tested, T. terrestris enzymes are comparable to, or in some cases better than, those from mesophilic strains. However, the differences are not so great as to compensate for the much lower productivity of T. terrestris compared to the improved T. reesei and Penicillium sp. strains. |
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Keywords: | Cellulose cellulase hemicellulase thermophilic fungi |
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