Enzymic interesterification of fats: Factors influencing the choice of support for immobilized lipase |
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| Authors: | R.A. Wisdom P. Dunnill M.D. Lilly A. Macrae |
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| Affiliation: | Department of Chemical and Biochemical Engineering, University College London, Torrington Place, London, UK;Unilever Research, Colworth House, Sharnbrook, UK |
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| Abstract: | Three grades of diatomaceous earth (Celite 560, Filtercel and Hyflo Supercel) and a controlled-pore silica have been examined for their suitability as support materials for lipase (triacyglycerol acylhydrolase, EC 3.1.1.3) catalysing the interesterification of fats. The controlled-pore silica gave a preparation with a low activity. Although all three Celites gave preparations with similar lipolytic activities, Hyflo Supercel gave the highest interesterification activity. The distribution of enzyme protein in Hyflo Supercel was examined by transmission electron microscopy. |
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| Keywords: | Support lipase interesterification diatomite |
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