Semicontinuous cellulase production in an aqueous two-phase system with Trichoderma reesei rutgers C30 |
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Authors: | Ingrid Persson Folke Tjerneld Bärbel Hahn-Hägerdal |
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Affiliation: | 1. Department of Biochemistry, Chemical Center, Lund University, P.O. Box 740, S-220 07 Lund, Sweden;2. Department of Applied Microbiology, Chemical Center, Lund University, P.O. Box 740, S-220 07 Lund, Sweden |
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Abstract: | Cellulases [see 1,4(1,3;1,4)-β-d-glucan 4-glucanohydrolase, EC 3.2.1.4] from Trichoderma reesei, Rutgers C30, can be semicontinuously produced in an aqueous two-phase system composed of dextran and poly(ethylene glycol) using Solka Floc BW 200 as substrate. When substrate was intermittently added along with fresh top phase, which replaced the withdrawn top phase containing the produced enzymes, a yield of 1740 U endo-β-d-glucanase/g cellulose and 59.3 FPU/g cellulose was extracted with the top phase. Without fresh substrate added, a yield of 3920 U endo-β-d-glucanase/g cellulose and 127.7 FPU/g cellulose was extracted after five runs. |
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Keywords: | Cellulose cellulase aqueous two-phase system semicontinuous culture |
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