Effects of water on equilibria catalysed by hydrolytic enzymes in biphasic reaction systems |
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Authors: | P.J. Halling |
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Affiliation: | Department of Bioscience and Biotechnology, University of Strathclyde, 204 George Street, Glasgow G1 1XW, UK |
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Abstract: | In the literature on hydrolase-catalysed synthetic reactions in aqueous-organic biphasic systems, it has been stated that low water concentrations contribute to favourable shifts in equilibrium. It is argued here that this is not a sufficient condition for mass action effects of water. A simple method of treating such equilibria is suggested, using the thermodynamic activity of water. Only when its activity is significantly reduced below 1 can water contribute to a shift of equilibrium in favour of synthetic products. Such a reduction is not necessarily obtained by creating a biphasic system, however low the water content, but requires that the aqueous phase becomes a very concentrated solution/dispersion of hydrophilic species. |
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Keywords: | Hydrolases biphasic systems equilibrium shift water activity |
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