Excised group II introns in yeast mitochondria are lariats and can be formed by self-splicing in vitro |
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| Authors: | R van der Veen A C Arnberg G van der Horst L Bonen H F Tabak L A Grivell |
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| Affiliation: | 2. Biochemical Laboratory State University Nijenborgh 16 9743 AG Groningen, The Netherlands;3. Department of Biology University of Ottawa 30 Somerset Street E Ottawa, Ontario, Canada K1N 6N5;1. Laboratory of Structural Biochemistry, Freie Universität Berlin, Takustraße 6, 14195 Berlin, Germany;2. Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany;1. Protein-Nucleic Acid Interaction Section, Structural Biophysics Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA;2. Structural Biophysics Laboratory, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA;3. Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78229, USA;1. Center for RNA Biology and Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA;1. College of Chemistry and Materials Science, Sichuan Normal University, Chengdu, China;2. College of Resources and Environment, Chengdu University of Information Technology, Chengdu, China;3. Faculty of Chemistry, Sichuan University, Chengdu, China;1. Linda Crnic Institute for Down Syndrome and Department of Pharmacology, School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA;2. Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, CO 80203, USA;1. Université Côte d’Azur, CNRS, Institut de Chimie de Nice UMR 7272, 06108 Nice, France;2. Plant Chemistry, Organic and Bioorganic Synthesis Team, URAC23, Faculty of Sciences, B.P. 1014, GEOPAC Research Center, Mohammed V University, Rabat, Morocco;3. Département de Chimie, Université Paris Descartes, Sorbonne Paris Cité, UFR des Sciences Pharmaceutiques, 4 avenue de l’Observatoire & UFR Biomédicale des Saints Pères, 45 rue des Saints Pères, Paris Fr-75006, France;4. Université Côte d’Azur, INSERM U1065, Centre Méditerranéen de Médecine Moléculaire (C3M), Bâtiment ARCHIMED, 151 Route de Saint-Antoine de Ginestière, BP 2 3194, 06204 Nice Cedex 3, France;5. Mohamed VI Polytechnic University, UM6P, 43150 Ben Guerir, Morocco |
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| Abstract: | Excised group II introns in yeast mitochondria appear as covalently closed circles under the electron microscope. We show that these circular molecules are branched and resemble the lariats arising through splicing of nuclear pre-mRNAs in yeast and higher eukaryotes. One member of this intron class (aI5c in the gene for cytochrome c oxidase subunit I) is capable of self-splicing in vitro, giving correct exon-exon ligation and resulting in the appearance of both linear and lariat forms of the excised intron. Nuclease digestion of the latter molecules reveals the presence of a complex oligonucleotide with the probable structure AGU, which thus resembles the branch point formed in the spliceosome-dependent reactions undergone by nuclear pre-mRNAs. Unlike group I introns, this group II intron is not demonstrably dependent on GTP for self-splicing and circularization of the isolated, linear intron is not observed. A model accounting for these observations is presented. |
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