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Protoplast fusion between Lactobacillus casei and Lactobacillus acidophilus
Authors:Yeehn Yeeh  Young Bae Jo  Oh Chang Kwon
Affiliation:(1) Institute of Basic Sciences, Inje University, Obang-dong 18-3, 621-749 Kimhae, Korea;(2) Department of Microbiology, Pusan National University, Jangjun-dong 30, 609-735 Kumjung-gu, Pusan, Korea;(3) Department of Horticulture, Dong-A University, Hadan-dong 840, 604-714 Saha-gu, Pusan, Korea
Abstract:Summary From the fusion between Lactobacillus casei and Lactobacillus acidophilus, 8 fusants were selected: Four were able to ferment maltose, lactose, galactose and mannose, but two had greater abilities of acid production than parents. Increased values of up to 7.6–8 % in beta -galactosidase activity were obtained from two when compared to that of L. acidophilus, whereas another 2 had activities of 800 and 548 nmol/mg protein/min comparable to that of L casei giving a value of 400 nmol/mg protein/min in phospho-beta-galactosidase activity.
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