Antibiotics resistance of a red alga,Griffithsia japonica |
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Authors: | Yoo Kyung Lee Gynheung An In Kyu Lee |
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Institution: | (1) School of Biological Sciences, Seoul National University, 151-742 Seoul, Korea;(2) Division of Molecular and Life Science, Pohang University of Science and Technology, 790-784 Pohang, Korea |
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Abstract: | To identify antibiotics suitable for stable transformation, we tested the resistance of a red alga,Griffithsia japonica Okamura, to four commonly used antibiotics. Very young germlings, with 1;3 cells, that germinated from the tetraspores were
cultured for 40 d in a half PES medium containing kanamycin, streptomycin, hygromycin B, or phleomycin.G. japonica was highly sensitive to 1 μg mL-1of phleomycin and g mL-1of hygromycin B. However, it was resistant to kanamycin and low levels of streptomycin and hygromycin B. These results suggest
that resistance genes for phleomycin or hygromycin can be used as selectable markers for transformation of G.japonica. |
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Keywords: | antibiotics Griffithsia japonica red alga transformation |
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