Long-term gene expression in dividing and nondividing cells using SV40-derived vectors |
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Authors: | David S Strayer Lokesh Agrawal Pierre Cordelier Bianling Liu Jean-Pierre Louboutin Elena Marusich Hayley J McKee Carmen N NiGongyi Ren Marlene S Strayer |
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Institution: | (1) Department of Pathology and Cell Biology, Jefferson Medical College, Philadelphia, PA |
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Abstract: | Among the goals of gene therapy is long-term expression of delivered transgenes. Recombinant Tag-deleted SV40 vectors (rSV40s) are especially well suited for this purpose. rSV40s deliver transgene expression that endures
for extended periods of time in tissue culture and in vivo, in both dividing and nondividing cells. These vectors are particularly
effective in transducing some cell types that have been almost unapproachable using other gene delivery systems, such as quiescent
hematopoietic progenitor cells and their differentiated derivatives. Other cellular targets include neurons, brain microglia,
hepatocytes, dendritic cells, vascular endothelium, and others. Because rSV40s do not elicit neutralizing antibodies they
are useful for in vivo gene delivery in settings where more than one administration may be desirable. The key characteristics
of these vectors include their high production titers and therefore suitability for large cell pools, effectiveness in delivering
intracellular proteins, and untranslated RNAs, maintenance of transgene expression at constant levels for extended times,
suitability for constitutive or conditional promoters and for combinatorial gene delivery and ability to integrate into genomes
of both dividing and nondividing cells. |
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Keywords: | |
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