Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation |
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| Authors: | Sebela Marek Stosová Tat'ána Havlis Jan Wielsch Natalie Thomas Henrik Zdráhal Zbynek Shevchenko Andrej |
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| Affiliation: | Department of Biochemistry, Faculty of Science, Palacky University, Slechtitel? 11, 783 71 Olomouc, Czech Republic. sebela@prfholnt.upol.cz |
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| Abstract: | Conjugating bovine trypsin with oligosaccharides maltotriose, raffinose and stachyose increased its thermostability and suppressed autolysis, without affecting its cleavage specificity. These conjugates accelerated the digestion of protein substrates both in solution and in gel, compared to commonly used unmodified and methylated trypsins. |
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| Keywords: | Bovine trypsin Conjugate In‐gel digestion Oligosaccharide PMF |
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