Multiplex quantitative PCR for detection of lower respiratory tract infection and meningitis caused by <Emphasis Type="Italic">Streptococcus pneumoniae</Emphasis>, <Emphasis Type="Italic">Haemophilus influenzae</Emphasis> and <Emphasis Type="Italic">Neisseria meningitidis</Emphasis> |
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| Authors: | Guma MK Abdeldaim Kristoffer Strålin Jens Korsgaard Jonas Blomberg Christina Welinder-Olsson Björn Herrmann |
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| Institution: | 1.Section of Clinical Bacteriology, Department of Medical Sciences,Uppsala University,Uppsala,Sweden;2.Department of Infectious Diseases,?rebro University Hospital,?rebro,Sweden;3.Department of Chest Diseases,Aarhus University Hospital,Aalborg,Denmark;4.Section of Clinical Virology, Department of Medical Sciences,Uppsala University,Uppsala,Sweden;5.Department of Clinical Bacteriology,Sahlgrenska University Hospital,Gothenburg,Sweden |
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| Abstract: | Background
Streptococcus pneumoniae and Haemophilus influenzae cause pneumonia and as Neisseria meningitidis they are important agents of meningitis. Although several PCR methods have been described for these bacteria the specificity
is an underestimated problem. Here we present a quantitative multiplex real-time PCR (qmPCR) for detection of S. pneumoniae (9802 gene fragment), H. influenzae (omp P6 gene) and N. meningitidis (ctrA gene). The method was evaluated on bronchoalveolar lavage (BAL) samples from 156 adults with lower respiratory tract infection
(LRTI) and 31 controls, and on 87 cerebrospinal fluid (CSF) samples from meningitis patients. |
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