Phage display of RNase A and an improved method for purification of phages displaying RNases |
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| Authors: | Korn K Foerster H H Hahn U |
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| Institution: | Institut für Biochemie, Fakult?t für Biowissenschaften, Pharmazie und Psychologie, Universit?t Leipzig, Germany. |
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| Abstract: | Functional ribonuclease A was presented on the surface of the filamentous phage M13 by fusion to the minor coat protein. RNase activity of the fusion protein was shown by a zymogram assay. In addition, we established a modified method for preparing RNase-displaying phages without contaminating host RNases. |
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