Oxidative metabolism of hydralazine. Evidence for nitrogen centered radicals formation |
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Authors: | B K Sinha A G Motten |
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Affiliation: | Laboratory of Environmental Biophysics National Institute of Environmental Health Sciences P.O. Box 12233 Research Triangle Park, North Carolina 27709 USA |
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Abstract: | The oxidative metabolism of hydralazine, a hydrazine-containing hypotensive drug, has been studied using a spin-trapping technique. In the presence of Cu2+, Fe2+ and Fe3+, hydralazine rapidly forms a nitrogen-centered-DMPO adduct with aN = 15.0G, aβH = 16.7G and aβN = 2.55G. While catalase has a very small inhibitory effect, superoxide dismutase completely inhibits the formation of the DMPO adduct. Mass spectral analysis of the adduct indicates that the hydralazyl radical is trapped with DMPO. Human red blood cells also catalyze the formation of a nitrogen-centered-DMPO adduct, aN = 15.9G, aβH = 19.4G and aβN = 1.7G, which is different than that obtained with metal ions. DMPO-H adduct is also formed in the red cells from hydralazine. |
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Keywords: | To whom all correspondence should be addressed. |
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