Histochemical localization of cysteine-rich proteins by tissue printing on nitrocellulose |
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| Authors: | R F Pont-Lezica J E Varner |
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| Affiliation: | Department of Biology, Washington University, St. Louis, Missouri 63130. |
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| Abstract: | A rapid technique for the histochemical localization of cysteine-rich proteins in plant tissues was developed. It is based on the immediate transfer of proteins to nitrocellulose membranes when a fresh cut organ is pressed against the membrane surface. The print was labeled for cysteine-rich proteins by reduction and alkylation of cysteinyl residues with dansylated iodoacetamide [N-iodoacetyl-N'-(-5-sulfo-1-naphthyl)ethylenediamine]. The S-carboxymethylated proteins were visualized by their fluorescence when excited with 360 nm light. |
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