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Avian Influenza Virus (H11N9) in Migratory Shorebirds Wintering in the Amazon Region,Brazil
Authors:Jansen de Araujo  Severino M. de Azevedo Júnior  Nicolas Gaidet  Renata F. Hurtado  David Walker  Luciano M. Thomazelli  Tatiana Ometto  Marina M. M. Seixas  Roberta Rodrigues  Daniele B. Galindo  Adriana C. S. da Silva  Arlinéa M. M. Rodrigues  Leonardo L. Bomfim  Marcelo A. Mota  Maria E. Larrazábal  Joaquim O. Branco  Patricia Serafini  Isaac S. Neto  John Franks  Richard J. Webby  Robert G. Webster  Edison L. Durigon
Abstract:Aquatic birds are the natural reservoir for avian influenza viruses (AIV). Habitats in Brazil provide stopover and wintering sites for water birds that migrate between North and South America. The current study was conducted to elucidate the possibility of the transport of influenza A viruses by birds that migrate annually between the Northern and Southern Hemispheres. In total, 556 orotracheal/cloacal swab samples were collected for influenza A virus screening using real-time RT-PCR (rRT-PCR). The influenza A virus-positive samples were subjected to viral isolation. Four samples were positive for the influenza A matrix gene by rRT-PCR. From these samples, three viruses were isolated, sequenced and characterized. All positive samples originated from a single bird species, the ruddy turnstone (Arenaria interpres), that was caught in the Amazon region at Caeté Bay, Northeast Pará, at Ilha de Canelas. To our knowledge, this is the first isolation of H11N9 in the ruddy turnstone in South America.
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