Dissection and Downstream Analysis of Zebra Finch Embryos at Early Stages of Development |
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Authors: | Jessica R. Murray Monika E. Stanciauskas Tejas S. Aralere Margaret S. Saha |
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Affiliation: | 1.Department of Biology, College of William and Mary |
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Abstract: | The zebra finch (Taeniopygiaguttata) has become an increasingly important model organism in many areas of research including toxicology1,2, behavior3, and memory and learning4,5,6. As the only songbird with a sequenced genome, the zebra finch has great potential for use in developmental studies; however, the early stages of zebra finch development have not been well studied. Lack of research in zebra finch development can be attributed to the difficulty of dissecting the small egg and embryo. The following dissection method minimizes embryonic tissue damage, which allows for investigation of morphology and gene expression at all stages of embryonic development. This permits both bright field and fluorescence quality imaging of embryos, use in molecular procedures such as in situ hybridization (ISH), cell proliferation assays, and RNA extraction for quantitative assays such as quantitative real-time PCR (qtRT-PCR). This technique allows investigators to study early stages of development that were previously difficult to access. |
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Keywords: | Developmental Biology, Issue 88, zebra finch (Taeniopygiaguttata), dissection, embryo, development, in situ hybridization, 5-ethynyl-2’ -deoxyuridine (EdU) |
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