The assay of dipeptides using fluorescamine and its application to determining dipeptidase activity. |
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Authors: | D Perrett J P Webb D B Silk M L Clark |
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Affiliation: | 1. The Department of Medicine, St. Bartholomew''s Hospital, London, England;2. The Department of Gastroenterology, St. Bartholomew''s Hospital, London, England |
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Abstract: | The differing effect of pH on the reaction of fluorescamine with amino acids and peptides has been utilized to develop a method for the quantitative estimation of peptides in the presence of amino acids. This method has been applied to the measurement of dipeptidase activities in human jejunal aspirates. |
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