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The assay of dipeptides using fluorescamine and its application to determining dipeptidase activity.
Authors:D Perrett  J P Webb  D B Silk  M L Clark
Institution:1. The Department of Medicine, St. Bartholomew''s Hospital, London, England;2. The Department of Gastroenterology, St. Bartholomew''s Hospital, London, England
Abstract:The differing effect of pH on the reaction of fluorescamine with amino acids and peptides has been utilized to develop a method for the quantitative estimation of peptides in the presence of amino acids. This method has been applied to the measurement of dipeptidase activities in human jejunal aspirates.
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