Monoclonal antibodies to the two most basic papaya proteinases |
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Authors: | Peter W. Goodenough Peter J. Kilshaw Fiona McEwan A. Jane Owen |
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Affiliation: | (1) AFRC Institute of Food Research, Reading Laboratory, Shinfield, RG2 9AT Reading |
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Abstract: | The proteinases fromCarica papaya include papain, isoenzymes of chymopapain and two proteinases A and B distinguished by their unusually high pI. The identity of one of the most basic proteinases has been questioned. The present report describes the preparation and characterisation of two monoclonal antibodies that react specifically with papaya proteinases A and B respectively and a third that identifies a common structural feature found in papain and proteinase A. |
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Keywords: | Carica papaya Caricaceae proteinase monoclonal antibodies |
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