The kinetics of coupled enzyme reactions. Applications to the assay of glucokinase, with glucose 6-phosphate dehydrogenase as coupling enzyme |
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| Authors: | Andrew C. Storer and Athel Cornish-Bowden |
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| Affiliation: | Department of Biochemistry, University of Liverpool, Liverpool L69 3BX, U.K. |
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| Abstract: | 1. A theory is developed to account for the kinetics of coupled-enzyme reactions without assuming that the second reaction follows first-order kinetics. 2. A simple procedure is described for applying the theory to the practical design of enzyme assays. 3. The validity of the theory is confirmed for the assay of glucokinase with glucose 6-phosphate dehydrogenase as coupling enzyme. 4. The possibility of extending the theory to three or more coupled reactions is discussed. |
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