Characterization of recombinant Desulfovibrio gigas ferredoxin. |
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| Authors: | P Rodrigues F Gra?a A L Macedo I Moura J J Moura |
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| Institution: | Departamento de Química, Centro Quìmica Fina e Biotecnologia, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2825 Monte de Caparica, Portugal. |
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| Abstract: | Dg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a 3Fe-4S] cluster per monomer. EPR and (1)H NMR data of recombinant and wild-type protein are compared. |
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