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| 中间丝蛋白Nestin在不同病程糖尿病大鼠肾组织中的表达 | |
| 引用本文： | 刘巍,段惠军,张悦,张怡,郝军. 中间丝蛋白Nestin在不同病程糖尿病大鼠肾组织中的表达[J]. 中国组织化学与细胞化学杂志, 2011, 20(4): 301-305. DOI: 10.3870/zgzzhx.2011.04.002 |
| 作者姓名： | 刘巍 段惠军 张悦 张怡 郝军 |
| 作者单位： | 1. 河北医科大学病理学教研室 2. 河北医科大学临床诊断学教研室,石家庄,050017 3. 河北省保定市传染病医院,保定,070000 |
| 基金项目： | 河北省卫生厅医学科学研究重点课题计划 |
| 摘 要： | 目的检测中间丝蛋白Nestin(巢蛋白)在不同病程糖尿病大鼠肾组织中的表达,探讨Nestin表达变化与糖尿病肾病发生发展的关系。方法腹腔注射链脲佐菌素(STZ)复制糖尿病(DM)大鼠模型,分别于第2、4、8、12和16周检测血糖、血尿素氮及24h尿蛋白量,HE染色观察肾脏病理学改变,免疫组织化学及流式细胞术检测Nestin表达水平。结果 HE染色可见,与对照组相比,DM组大鼠从第2周起出现肾小球体积增大;至8周时系膜基质明显增多,系膜区增宽;12、16周时肾小球呈分叶状,肾小管上皮细胞可见明显空泡变性及坏死。免疫组织化学和流式细胞术结果显示,DM各组Nestin表达水平均高于正常对照组,且在第8周时达高峰,而后逐渐下降。结论在不同病程糖尿病大鼠模型中,中间丝蛋白Nestin的表达先升高,而后降低,可能参与了糖尿病肾损害的发生与发展。 |
| 关 键 词： | 糖尿病肾病 巢蛋白 中间丝 |
Expression of intermediate filament protein Nestin in renal tissue of diabetic rats |
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| Liu Wei,Duan Huijun,Zhang Yue,Zhang Yi,Hao Jun. Expression of intermediate filament protein Nestin in renal tissue of diabetic rats[J]. Chinese Journal of Histochemistry and Cytochemistry, 2011, 20(4): 301-305. DOI: 10.3870/zgzzhx.2011.04.002 | |
| Authors: | Liu Wei Duan Huijun Zhang Yue Zhang Yi Hao Jun |
| Affiliation: | Liu Wei1,Duan Huijun1*,Zhang Yue2,Zhang Yi3,Hao Jun1(1Department of Pathology,2Department of Clinical Diagnostics,Hebei Medical University,Shijiazhuang 050017,3Baoding Infectious Disease Hospital,Baoding 070000,China) |
| Abstract: | Objective To explore the expression of nestin in diabetic rats at different states,and to investigate the relationship between the changes of nestin and diabetic nephropathy.Methods The diabetic rat model was induced by intraperitoneal injection of streptozotocin(STZ).Rats were killed at 2,4,8,12 and 16 weeks of experiment.Blood glucose,serum urea nitrogen and 24-hour urine protein were examined by biochemistry.The pathological changes of kidneys were detected by HE staining.The expression of nestin protein... |
| Keywords: | Diabetic nephropathy Nestin Intermediate filament |
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