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| 细胞核内特征值的改变可引起DNA倍体的变化 | |
| 引用本文： | 王景洁,孙全新,孙小蓉,牛晓泉.细胞核内特征值的改变可引起DNA倍体的变化[J].中国组织化学与细胞化学杂志,2011,20(4):367-371. |
| 作者姓名： | 王景洁 孙全新 孙小蓉 牛晓泉 |
| 作者单位： | 1. 武汉市第五医院保健科,武汉,430050 2. 湖北中山医院细胞室,武汉,430033 3. 华中科技大学同济医学院中加肿瘤早期诊断研究中心,武汉,430022 4. 武汉市第五医院科教科,武汉,430050 |
| 摘 要： | 目的通过测定不同DNA倍体细胞,研究细胞核内特征值的改变。方法用宫颈刷刷出宫颈细胞,经固定后,用涂片离心机制成二张玻片,一张行巴氏染色作TBS诊断,另一张行Feulgen染色做DNA定量测定。通过对宫颈细胞核图像内像素的统计,计算出细胞核内多种特征值,比较不同DNA倍体细胞内特征值的不同。结果 161873例妇女行宫颈细胞学检查,常规细胞学检查发现2454例低级别鳞状上皮内病变(low-grade squamous intraepithelial lesion,LSIL)和523例高级别鳞状上皮内病变(high-grade squamous intraepithelial lesion,HSIL);而DNA倍体分析发现3412例有3个以上>5c细胞。84%以上的LSIL和HSIL病例均可见倍体异常细胞。与2c细胞相比,4c、5c、7c及9c细胞核面积及核半径明显增大;7c、9c细胞核内平均光学密度和紧实度均值也有明显改变,而光密度方差和灰度熵无变化。结论宫颈细胞DNA倍体改变往往伴有细胞形态和DNA核内分布等特征值的改变。 |
| 关 键 词： | DNA倍体 图像分析系统 宫颈癌 非整倍体细胞 |
Changes of nuclear features with different DNA ploid |
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| Wang Jingjie,Sun Quanxin,Sun Xiaorong,Niu Xiaoquan.Changes of nuclear features with different DNA ploid[J].Chinese Journal of Histochemistry and Cytochemistry,2011,20(4):367-371. | |
| Authors: | Wang Jingjie Sun Quanxin Sun Xiaorong Niu Xiaoquan |
| Institution: | Wang Jingjie1,Sun Quanxin2,Sun Xiaorong3,Niu Xiaoquan4*(1Department of Health Care,Wuhan 5th Hospital,Wuhan 430050,2Department of Cytology,Hubei Zhongshan Hospital,Wuhan 430033,3China-Canada Early Cancer Detection Research Center,Tongji Medical College,Huazhong University of Science & Technology,Wuhan 430022,4Department of Education,China) |
| Abstract: | Objective To study the nuclear features in different DNA ploids of cervical cells by DNA image cytometry.Methods Cervical cells were collected by a brush and fixed.Two slides per sample were made by a cytospin.One slide was stained by Pap solution for TBS diagnosis,whice the other one was stained by Feulgen for DNA ploidy analysis.Nuclear features,such as nuclear area and mean optical density(OD),were also measured.Results Cervical samples from 161,873 women were examined.2454 low-grade squamous intraepithe... |
| Keywords: | DNA ploid Image cytometer Cervical cancer Aneuploid cell |
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