| 抑制自噬促进冬凌草甲素诱导的多发性骨髓瘤细胞凋亡涉及胞内ROS产生 |
| |
| 引用本文： | 曾蓉,陈燕,崔国惠.抑制自噬促进冬凌草甲素诱导的多发性骨髓瘤细胞凋亡涉及胞内ROS产生[J].中国组织化学与细胞化学杂志,2011,20(4):295-300. |
| |
| 作者姓名： | 曾蓉 陈燕 崔国惠 |
| |
| 作者单位： | 华中科技大学附属协和医院血液病研究所,湖北,武汉,430022 |
| |
| 摘 要： | 目的本实验主要研究冬凌草甲素诱导多发性骨髓瘤发生自噬、凋亡,两者之间的关系以及所涉及的相关机制。方法利用MTT比色法检测冬凌草甲素对多发性骨髓瘤RPMI8226细胞的增殖活性影响;透视电镜观察细胞内凋亡和自噬的形态学改变;TUNEL检测细胞凋亡;分别利用以下技术检测处理后的细胞内的自噬变化:使用QDs605nm-Anti-LC3荧光探针以及免疫荧光技术定位细胞胞内LC3Ⅰ和LC3Ⅱ蛋白,利用western blot免疫印记技术检测Beclin 1蛋白表达水平;利用DCFH-DA探针以及流式细胞术检测细胞胞内ROS水平。结果冬凌草甲素能明显抑制RPMI8226细胞增殖,其抑制作用呈时间、剂量依赖性;冬凌草甲素能同时诱发细胞凋亡、自噬和胞内ROS产生;NAC完全抑制胞内ROS产生后冬凌草甲素诱导的细胞凋亡消失;3-MA抑制自噬后,冬凌草甲素诱导的胞内ROS产生进一步增多,凋亡增多。结论冬凌草甲素能明显抑制RPMI8226细胞增殖;冬凌草甲素同时诱发细胞凋亡和自噬;胞内ROS产生介导冬凌草甲素诱导的凋亡;凋亡为细胞死亡的主要途径,而自噬通过下调胞内ROS产生抑制凋亡。
|
| 关 键 词： | 冬凌草甲素 凋亡 自噬 ROS 多发性骨髓瘤 |
Inhibition of autophagy enhances apoptosis involved with intracellular ROS generation in multiple myeloma cells exposed to Oridonin |
| |
| Zeng Rong,Chen Yan,Cui Guohui.Inhibition of autophagy enhances apoptosis involved with intracellular ROS generation in multiple myeloma cells exposed to Oridonin[J].Chinese Journal of Histochemistry and Cytochemistry,2011,20(4):295-300. |
| |
| Authors: | Zeng Rong Chen Yan Cui Guohui |
| |
| Institution: | Zeng Rong,Chen Yan,Cui Guohui*(Union Hospital,Huazhong University of science and technology.Wuhan Hubei,430022 China) |
| |
| Abstract: | Objective To explore the oridonin-induced apoptosis and autophagy of multiple myeloma cells the relationship between them,and the involved molecular mechanisms.Methods RPMI8226 cell vitality was assessed by MTT assay.The morphology of apoptosis and autophagy was observed by TEM.TUNEL assay was used to determine apoptosis.The LC3 localization and the Beclin1 protein level indicating autophagy level were analyzed by immunofluorescence analysis using the QDs605nm-Anti-LC3 fluorescent probe and western blot ass... |
| |
| Keywords: | ROS |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
