Probiotic inhibits the cytopathic effect induced by Escherichia coli O157:H7 in Vero cell line model |
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Authors: | Tahamtan Y Kargar M Namdar N Rahimian A Hayati M Namavari M M |
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Institution: | 1. Razi Vaccine and Serum Research Institute, Shiraz, Iran;2. Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran |
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Abstract: | Aims: The effectiveness of four strains of Bifidobacteria against enterohemorrhagic Escherichiacoli O157:H7 infection was studied using a Vero cell model. Methods and results: E. coli O157 was inoculated on the Vero cell line before and after treatment with probiotic. The cytopathic effect (CPE) was evaluated during 24 h of incubation. The results indicated that Shiga toxin activity was inhibited by the probiotic. To prevent a Stx2 CPE, the probiotic needs one log more than the Stx1. Conclusion: The Vero cell assay, in particular, is a good model to evaluate the effect of Bifidobacteria inhibiting bacterial attachment because of soluble substances and the competitive aspect and could be used in a variety of foods like milk and yoghurt to protect pathogen bacteria. Significance and Impact of the Study: Probiotics could control pathogenic bacteria and Vero cell introduce as a model for evaluation of probiotics against pathogen bacteria. |
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Keywords: | E coli O157:H7 probiotic Vero cell |
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