Alkyl-substituted methoxysilanes enhance the activity and stability of d-amino acid oxidase encapsulated in biomimetic silica |
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Authors: | I-Ching Kuan Chiao-An Chuang Shiow-Ling Lee Chi-Yang Yu |
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Affiliation: | Department of Bioengineering, Tatung University, 40 Chungshan N. Rd. Sec. 3, Taipei, 10452, Taiwan. iching@ttu.edu.tw |
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Abstract: | Several alkyl-substituted methoxysilanes were evaluated as potential activity and stability enhancing agents for biomimetic silicification of Rhodosporidium toruloides D-amino acid oxidase (RtDAO). When methyl-substituted silanes along with tetramethoxysilane were used as silicic acid precursors for polyallylamine (PAA)--or R5 peptide-catalyzed silicic encapsulation, the RtDAO activity increased with the degree of substitution and the molar ratio up to 15 % of methyl-substituted silanes added. In the presence of 15 mol% trimethylmethoxysilane, the specific activities of encapsulated RtDAO catalyzed by PAA and R5 increased by 1.4- and 4.8-fold, respectively. For PAA-catalyzed encapsulation, a 2.4-fold increase occurred with 30 mol% n-propyltrimethoxysilane; this modification increased the T (m) value by 10 °C and gave a threefold longer half-life in the presence of 10 mM H(2)O(2) as compared to the encapsulation using tetramethoxysilane only. |
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