Determination of seven prostanoids in 1 ml of urine by gas chromatography—negative ion chemical ionization triple stage quadrupole mass spectrometry |
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Authors: | Horst Schweer Bernhard Watzer Hannsjrg W Seyberth |
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Institution: | Horst Schweer, Bernhard Watzer,Hannsjörg W. Seyberth |
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Abstract: | In an isotope dilution assay, prostaglandin (PG) E2, 6-keto-PGF1α, thromboxane (Tx) B2 and their metabolites PGE-M (11α-hydroxy-9,15-dioxo-2,3,4,5,20-pentanor-19-carboxyprostanoic acid), 2,3-dinor-6-keto-PGF1α, 2,3-dinor-TxB2 and 11-dehydro-TxB2 were determined in urine by gas chromatography—triple stage quadrupole mass spectrometry (GC—MS—MS). After addition of deuterated internal standards, the prostaglandins were derivatized to their methoximes and extracted with ethyl acetate—hexane. The sample was further derivatized to the pentafluorobenzylesters and purified by thin-layer chromatography (TLC). Three zones were scraped from the TLC plate. The prostanoid derivatives were converted to their trimethylsilyl ethers and the products were quantified by GC—MS—MS. In each run, two or three prostanoids were determined. |
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