Establishment of duck cell line derived from experimental tumor induced by 20-methylcholanthrene |
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Authors: | Chil-Yong Kang John A Shadduck |
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Institution: | (1) Department of Microbiology, The University of Texas Health Science Center, Southwestern Medical School, 75235 Texas, Dallas |
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Abstract: | Summary Experimental tumors developed in white Pekin ducks after intramuscular implantation of 20-methylcholanthrene. Cells derived
from the primary tumor were adapted successfully to grow in vitro and have growth characteristics similar to that of established
cell lines of mammalian origin. The cell density rises rapidly and the doubling time is approximately 19 hr. The duck cells
have been cultured succesfully for at least 80 passages in vitro. The continuously cultured cells have the characteristic
chromosome pattern of duck, and the DNA of the duck cell line hybridized with duck liver DNA. We believe we have established
a continuous cell line of avian origin. Electron-microscopic examinations of the tumor cells and RNA-directed DNA polymerase
of the cell-free supernate show no evidence of endogenous virus production.
This study was supported by Public Health Service Research Grants CA 16479 and CA 20012 from the National Cancer Institute
and RR 00890 from the National Institutes of Health. |
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Keywords: | experimental tumors ducks 20-methylcholanthrene cell line |
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