(1) Department of Non-infectious diseases, Faculty of Veterinary Medicine, Lithuanian Veterinary Academy, Kaunas, Lithuania;(2) Department of Production Animal Medicine, University of Helsinki, Saarentaus, Finland
Abstract:
Background
Several studies have been published where sperm plasma membrane integrity correlated to fertility. In this study we describe a simple fluorometer-based assay where we monitored the fluorescence intensity of artificially membrane-ruptured spermatozoa with a fixed time staining with fluorescent DNA dyes.