C-nuclear magnetic resonance studies of the biosynthesis of 5-aminolevulinic acid destined for chlorophyll formation in dark-grown Scenedesmus obliquus |
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Authors: | Tamiko Oh-Hama Haruo Seto Shigetoh Miyachi |
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Abstract: | The 13C-nuclear magnetic resonance (NMR) spectra of chlorophyll a formed in dark-grown Scenedesmus obliquus (Turp.) Kützing in the presence of [1-13C]glutamate, [2-13C]- and [1-13C]glycineshowed that the 13C of glutamate was specifically incorporated into the eight-carbon atoms in the tetrapyrrole macrocycles derived from C-5 of 5-aminolevulinic acid (ALA), while the C-2 of glycine was only incorporated into the methyl carbon of the methoxycarbonyl group attached to the isocyclic ring of chlorophyll a. No specific enrichment of these nine carbon atoms was observed in the spectrum of chlorophyll a formed in the presence of [1-13C]-glycine. These labeling patterns provide evidence for the operation of the C5-pathway and against the operation of the ALA synthase pathway for chlorophyll formation in darkness. |
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Keywords: | biosynthesis of 5-aminolevulinic acid chlorophyll biosynthesis 13C-nuclear magnetic resonance Scenedesmus obliquus |
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