Structural Analysis of a recA-like Gene in the Genome of Arabidopsis thaliana |
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Authors: | Sato Shusei; Hotta Yasuo; Tabata Satoshi |
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Institution: | 1Kazusa DNA Research Institute 1532-3 Yana-Uchino, Kisarazu, Chiba 292 Japan
2Nara Institute of Science and Technology 8916-5 Takayama-cho, Ikoma-shi, Nara 630-01 Japan |
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Abstract: | A recA-like gene was identified in the genome of Arabidopsisthaliana by means of PCR using primers designed on the basisof previously reported amino acid sequences of eukaryotic RecA-likeproteins. The structure of the gene, termed ArLIM15, was investigatedby comparing the primary structure of the genomic DNA with thatof the corresponding cDNA. The open reading frame, which wassplit into 15 exons, was established to have the capacity forencoding a 37.3-kDa polypeptide. The amino acid sequence ofthe putative product of ArLIM15 showed a high degree of similaritytothat of LIM15 in the monocotyledonous plant Lilium, includinga 93% identity, and to those of other recA-like genes in yeastsand vertebrates with identities of 6971%. Phylogeneticanalysis indicated ArLIM15 to be much closer to meiosis-specificLIM15 and DMC1 in Saccharomyces cerevisiae than to RAD51 inS. cerevisiae and its homologues on an evolutionary scale. |
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Keywords: | recA-like gene recombination Arabidpsis thaliana |
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