Oligodeoxyadenylate stimulates the protein kinase activity of anti-DNA sIgA from human milk |
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Authors: | Kit Yuri Kuligina Elena Semenov Dimitry Richter Vladimir |
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Institution: | Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv. kit@biochem.viv.ua |
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Abstract: | Preparations of anti-DNA sIgA were obtained from human milk by sequential chromatography on protein A-sepharose, DEAE-fractogel and DNA-cellulose. The influence of oligonucleotides on protein kinase activity was investigated. It was discovered that incubation of anti-DNA sIgA with oligodeoxyriboadenylate d(A)12 stimulates the phosphorylation of polypeptides of sIgA in the presence of gamma-32P]ATP. The greatest was the incorporation of P into the sIgA H-chains. We also demonstrated stimulation of the casein kinase activity of anti-DNA sIgA by d(A)12. The stimulation of the protein kinase activity of anti-DNA sIgA by oligoriboadenylate r(A)12 was not detected. |
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