Mitochondrial chromatin in Paramecium aurelia |
| |
Authors: | E. Olszewska and A. Tait |
| |
Affiliation: | (1) Department of Genetics, University of Edinburgh, Edinburgh, Scotland |
| |
Abstract: | Summary Chromatin-like structures have been observed in material extracted from the mitochondria of Paramecium aurelia and evidence is presented which establishes that these structures do not originate from nuclear contamination of mitochondrial preparations but are exclusively of mitochondrial origin. Extraction of these chromatin-like structures with dilute acid followed by gel electrophoresis of the extract shows the presence of five major basic proteins which are of similar electrophoretic mobility to histones. Digestion of mitochondrial extracts with endogenous nuclease, followed by electrophoresis of the extracted DNA, demonstrated that the mitochondrial genome is protected from nuclease digestion by regular repeating units very similar to those observed in the nuclease digestion of chromatin.This research was supported by a postgraduate studentship from University of Edinburgh to E. Olszewska and by the Science Research Council. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|