| 海洋链霉菌Streptomyces sp. SCSIO 1672及其代谢产物水杨酸的分离鉴定 |
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| 引用本文： | 罗明和,汪中文,黄洪波,朱清华,田新朋,白志川,张偲,张长生,鞠建华.海洋链霉菌Streptomyces sp. SCSIO 1672及其代谢产物水杨酸的分离鉴定[J].微生物学杂志,2010,30(6):22-26. |
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| 作者姓名： | 罗明和 汪中文 黄洪波 朱清华 田新朋 白志川 张偲 张长生 鞠建华 |
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| 作者单位： | [1]西南大学园艺园林学院,重庆400700 [2]中国科学院海洋生物资源可持续利用重点实验室,广东广州510301 [3]广东省海洋药物重点实验室,广东广州510301 [4]中国科学院南海海洋研究所,广东广州510301 |
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| 基金项目： | 中国科学院知识创新工程重要方向项目，中国科学院南海海洋所领域前沿项目 |
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| 摘 要： | 从南海海洋沉积物中分离得到1株海洋放线菌,鉴定为链霉菌Streptomyces sp. SCSIO 1672。通过优化发酵条件,采用海虾生物致死活性和高效液相色谱追踪,利用有机溶剂萃取、正相硅胶、反相硅胶等各种色谱层析方法分离出活性化合物,通过波谱数据解析出海洋放线菌SCSIO 1672次级代谢产物中的该活性化合物为水杨酸。
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| 关 键 词： | 海洋放线菌 链霉菌 水杨酸 |
Isolation and Identification of a Marine Strain Streptomyces sp.SCSIO 1672 and Its Metabolite Salicylic Acid |
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| LUO Ming-he,WANG Zhong-wen,HUANG Hong-bo,ZHU Qing-hu,TIAN Xin-peng,BAI Zhi-chuan,ZHANG Si,ZHANG Chang-sheng,JU Jian-hua.Isolation and Identification of a Marine Strain Streptomyces sp.SCSIO 1672 and Its Metabolite Salicylic Acid[J].Journal of Microbiology,2010,30(6):22-26. |
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| Authors: | LUO Ming-he WANG Zhong-wen HUANG Hong-bo ZHU Qing-hu TIAN Xin-peng BAI Zhi-chuan ZHANG Si ZHANG Chang-sheng JU Jian-hua |
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| Institution: | 1.Coll.of Horticult.＆ Landscape Archit.,SW Uni.,Chongqing 400700;2.Key Lab.of Marine Bio-Res.Sustainable Util.,Guangzhou 510301;3.Guangdong Key Lab.of Marine Materia Medica,Guangzhou 510301;4.Ctr.for Marine Microorg.,South China Sea Inst.of Oceanology,Chinese Acad.of Sci.,Guangzhou 510301) |
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| Abstract: | A marine actinomycetes strain was isolated and identified as Streptomyces sp.1672 by 16S rDNA sequence analysis.Its fermentation condition was optimized and its bioactive compound was tested by means of HPLC analysis and bioassay-guided fractionation using brine shrimp toxicity test,and was isolated by solvent extraction,normal and reverse-phase silica gel and other chromatographies.The structure of the bioactive compound was identified as salicylic acid by spectral data analyses. |
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| Keywords: | marine actinomycetes Streptomyces sp salicylic acid |
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