1株白木香内生真菌中抗肿瘤活性成分的分离纯化和结构鉴定

采用活性追踪的方法对白木香内生真菌螺旋木霉Trichoderma spiraleA17的抗肿瘤活性代谢产物进行了分离纯化和结构鉴定。通过凝胶柱层析和反相硅胶柱层析,从其发酵液的活性组分中分离到2个无色针晶化合物,质谱和核磁共振的鉴定结果表明化合物1是一个八氢萘衍生物,命名为木霉酸(Trichodermic acid),化合物2是酪醇(Tyrosol)。活性实验显示化合物1对SF-268、MCF-7和NCI-H460 3种肿瘤细胞株都具有显著的增殖抑制活性,而化合物2对这3种肿瘤细胞株只有微弱的增殖抑制活性。

Isolation and Identification of Anti-Tumor Constituents from an Endophytic Fungus of Baimuxiang(Aquilaria sinensis)

Adopting activity trailing method,anti-tumor active metabolites from the endophytic fungus Trichoderma spirale A17 in officinal baimuxiang(Aquilaria sinensis) were isolated and identified.Column chromatography on Sephadex LH-20 and C18 silica gel was used,and two kinds of colorless needle crystallized compound were obtained from the active constituent of fermentation broth.Data analysis of mass spectrometry and nuclear magnetic resonance,indicated the compound 1 was an octo-hydrogen derivative of naphthalen...