Beyond the diffraction limit: far-field fluorescence imaging with ultrahigh resolution |
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Authors: | Rice James H |
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Affiliation: | School of Chemical Sciences and Pharmacy, University of East Anglia, Earlham Road, Norwich, UK NR2 3RG. james.rice@uea.ac.uk |
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Abstract: | Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution has stimulated research into developing methods to image beyond the diffraction limit based on far-field fluorescence microscopy techniques. Rapid progress is being made in this area of science with methods emerging that enable fluorescence imaging in the far-field to possess a resolution well beyond the diffraction limit. This review outlines developments in far-field fluorescence methods which enable ultrahigh resolution imaging and application of these techniques to biology. Future possible trends and directions in far-field fluorescence imaging with ultrahigh resolution are also outlined. |
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