山羊生长激素基因5调控区的多态性分析

以鲁北白山羊、引进波尔山羊、纯繁波尔山羊以及鲁北白山羊与波尔山羊的杂交一代、回交一代共计274个个体为研究材料，用两对引物分别扩增山羊生长激素(GH)基因5^'区的26－239bp以及225－429bp片段，扩增产物经SSCP分析发现均存在多态性。在26－239bp片段上，波尔山羊及杂交后代以 AA型个体占多数，而鲁北白山羊则BB型个体较多；在225－429bp片段上，所有种群均以 CC型个体较多。对两个片段的纯合型（AA，BB；CC，DD）分别克隆测序发现：（1）26－239bp片段上AA型在第60位发生了C→T的突变，第211位发生碱基C的丢失，（2）225－429bp片段上，DD型存在3处突变，分别为264位由T→C，292位由T→A，372位由C→T。上述结果为首次实验证实山羊生长激素5^'调控区存在序列多态性。

Polymorphism Analysis of the Goat Growth Hormone Gene in the 5';Regulatory Sequence

The polymorphism of goat growth hormone gene in 5'-region in 274 goats including Lubei white goat, introducted Boer goat, pure bred Boer goat , hybrid generation 1 of LuBei white goat and Boer goat , backcross of hybrid generation 1 and Boer goat were detected by PCR-SSCP with two different pairs of primers. The result showed that in 26-239bp most of the Boer goats and hybrid generation presented as AA genotype,while most LuBei white goats presented as BB genotype. In 225-429bp all breeds presented as more CC genotype.The amplified fragments of AA,BB and CC,DD of the two fragments were cloned and sequenced . The result showed that in 26-239bp AA genotype had one substitution mutation at 60 site (C-->T), and base C losing at 211 site and DD genotype had three substitution mutations at 264 site (T-->C), 292 site (T-->A) and 372 site (C-->T) in 225-429bp. The results above-mentioned first confirmed that there were polymorphisms in 5' region of GH gene.