| 鸭乙型肝炎病毒表面抗原表达质粒的构建及鸭血清中DHBsAg的初步检测 |
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| 引用本文： | 万大方,李秀珍.鸭乙型肝炎病毒表面抗原表达质粒的构建及鸭血清中DHBsAg的初步检测[J].病毒学报,1989,5(3):211-216. |
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| 作者姓名： | 万大方 李秀珍 |
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| 作者单位： | 上海市肿瘤研究所
(万大方，李秀珍，李宏年，顾建人，关赛方，陈复华，钱耕荪)，上海市肿瘤研究所(俞晔) |
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| 摘 要： |
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| 关 键 词： | DHBsAg 基因表达 血清学检测 |
CONSTRUCTION OF A DHBV preS/S EXPRESSION PLASMID AND ITS PRELIMINARY USE FOR THE DETECTION OF DHBsAg IN DUCK SERA |
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| Wan Dafang Li Xiuzheng LI Hongnian Gu Jianren,Guaa Saifang Chen Fuhua Qian Gengxun Yu Yi.CONSTRUCTION OF A DHBV preS/S EXPRESSION PLASMID AND ITS PRELIMINARY USE FOR THE DETECTION OF DHBsAg IN DUCK SERA[J].Chinese Journal of Virology,1989,5(3):211-216. |
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| Authors: | Wan Dafang Li Xiuzheng LI Hongnian Gu Jianren Guaa Saifang Chen Fuhua Qian Gengxun Yu Yi |
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| Institution: | Shanghai Cancer Institute |
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| Abstract: | A DHBV preS/S expression plasmid(D F.W.29)was constructed with vector PEX34a. SDS-PAGE and laser scanning showed a unique band of fusion protein (MW 30000)accounting for 16% of total bacterial proteins.Antibody against the fusion protein was prepared by immunizing rabbits with the gel band and testified with Western blot analysis. The oc-currence of DHBsAg in duck serum samples was determined by Westera blot. Our data seem to be mostly consistent with those obtained by dot hybridization of DHBV-DNA. The antibody against the fusion protein therefore, can be usad to reveal DHBV carrier conditions and tin relationship between duck hepatitis B and liver cancer. |
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| Keywords: | DHBsAg Gene expression Serological assay |
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