Statement on the nomenclature of dog C4 allotypes |
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| Authors: | Gabriele Doxiadis Wolfram Schoen Ilias Doxiadis H. Joachim Deeg Goeffrey J. O'Neill Hans Grosse-Wilde |
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| Affiliation: | (1) Department of Immunogenetics, University Hospital of Essen, Essen, Germany;(2) The Vincent T. Lombardi Cancer Research Center, Georgetown University Medical Center, Washington, DC, USA;(3) Tissue Typing Laboratory, The Miami Blood Bank, Miami, Florida, USA |
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| Abstract: | As a collaborative work of three laboratories the polymorphism of the canine fourth complement component (C4) was studied in a total of 131 unrelated dogs from different breeds and mongrels. Using high voltage electrophoresis followed by an immunoblotting technique, we detected eight distinct variants. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of canine C4 showed an additional heterogeneity of the  and  chains which resulted in a total of 11 variants in the population studied. So that more precise information concerning the respective C4 allotypes will be available, a nomenclature is proposed designating not only the migration pattern of the C4 variants in agarose gels but also the heterogeneity of the C4 chains observed in SDS-PAGE.Abbreviations used in this paper AGE agarose gel electrophoresis - C4 fourth complement component - DLA dog leukocyte antigen - EDTA ethylene diaminetetraacetate - FCS fetal calf serum - PBS phosphate-buffered saline - PBST PBS and 0.22% Tween-20 - SDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresis |
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