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Eosin B |
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| Authors: | W C Holmes C G Melin A R Peterson |
| Institution: | a U. S. Department of Agriculture, Contribution No. 208, from the Color and Farm Waste Division, Bureau of Chemistry and Soils, Washington, DC |
| Abstract: | The two known isomeric dibromodinitrofiuoresceins have been prepared in a fairly pure state, and their absorption spectra determined.
Commercial samples of eosin B are not 4, 5-dibromo—2, 7-dinitrofluorescein, as stated in dye indices. They are mixtures which contain other bromonitro derivatives of fluorescein as well as di-bromodinitro derivatives. The color acid method provides a substantially reliable means of determining actual dye content with commercial samples of the dye, but the reduction method may prove decidedly misleading. |
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