Isolation and biochemical characterization of a zona pellucida-binding glycoprotein of boar spermatozoa. |
| |
| Authors: | V Jonákova L Sanz J J Calvete A Henschen D Cechová E T?pfer-Petersen |
| |
| Affiliation: | Institute of Molecular Genetics, Prague, Czechoslovakia. |
| |
| Abstract: | Lectin-like molecules on the sperm surface are implicated in the process of gamete recognition and adhesion. We have isolated and biochemically characterized a 15 kDa glycoprotein from ejaculated boar sperm which possess zona pellucida-binding- and haemagglutinating-activity. The zona/15 kDa protein interaction is inhibited by fucoidan, suggesting that the glycoprotein is one of the sperm components which participate in the initial gamete interaction. N-Terminal sequence analysis of the isolated 15 kDa glycoprotein showed that it may belong to the same sperm/egg recognition-mediating protein family as the sea urchin sperm protein binding. |
| |
| Keywords: | |
|
