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A more efficient method to generate integration-free human iPS cells |
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| Authors: | Okita Keisuke Matsumura Yasuko Sato Yoshiko Okada Aki Morizane Asuka Okamoto Satoshi Hong Hyenjong Nakagawa Masato Tanabe Koji Tezuka Ken-ichi Shibata Toshiyuki Kunisada Takahiro Takahashi Masayo Takahashi Jun Saji Hiroh Yamanaka Shinya |
| Institution: | Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. okita@cira.kyoto-u.ac.jp |
| Abstract: | We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ～20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future. |
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