Evaluation of the effect of <Emphasis Type="Italic">in vitro</Emphasis> stress and competition on tissue culture response of flax |
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Authors: | M Yildiz |
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Institution: | (1) Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, CZ - 370 05 eské Bud jovice, Czech Republic;(2) AGRITEC Ltd., Zem d lská 16, CZ - 787 01 umperk, Czech Republic |
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Abstract: | This study was carried out to investigate the in vitro competition in tissue culture of three flax (Linum usitatissimum L.) cultivars using different distances among hypocotyl explants cultured. Hypocotyl fresh and dry masses, shoot regeneration
percentage, shoot number per hypocotyl, regenerated shoot length and total chlorophyll content were examined during shoot
regeneration, while plantlet height, number of roots and length of roots were recorded during rooting. With decreasing distance
among explants we observed increased shoot regeneration and rooting till a certain point from where stress initiated and significant
decreases in all parameters observed. Explants cultured at distance 1.0 cm were found to be at their optimum. |
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