| NGX6基因对人结肠癌细胞HT-29细胞周期的影响 |
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| 引用本文： | 王晓艳,沈守荣,刘 芬,李晓玲,范松青.NGX6基因对人结肠癌细胞HT-29细胞周期的影响[J].生物化学与生物物理进展,2006,33(1):45-50. |
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| 作者姓名： | 王晓艳 沈守荣 刘 芬 李晓玲 范松青 |
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| 作者单位： | 1. 中南大学湘雅三医院消化内科,长沙,410013
2. 中南大学湘雅医学院肿瘤研究所,长沙,410078 |
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| 基金项目： | 中国科学院资助项目;浙江省湖州市自然科学基金 |
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| 摘 要： | NGX6基因是新克隆的候选抑瘤基因,研究表明NGX6重表达可抑制结肠癌细胞的增殖.为进一步研究NGX6对细胞周期的影响,采用流式细胞仪检测NGX6重表达对结肠癌细胞HT-29细胞周期的影响,发现NGX6重表达可增加HT-29细胞在G0/G1期的分布比例,减少了S,G2,M期细胞数.利用蛋白质印迹和流式细胞术分析NGX6转染前后HT-29细胞周期素(cyclins)和细胞周期素依赖性蛋白激酶抑制物(cyclin-dependentkinaseinhibitor,CKI)的表达变化,发现NGX6可下调HT-29细胞中cyclinE、cyclinD1的表达及上调p27的表达,对cyclinA和cyclinB的表达无明显影响,p16在三组结肠癌细胞中均无表达.研究结果表明,NGX6在HT-29细胞中通过下调cyclinE、cyclinD1和上调p27的表达,阻滞细胞周期于G0/G1期,从而发挥其在结肠癌中的抑瘤作用.
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| 关 键 词： | NGX6 HT-29细胞 细胞周期 细胞周期素 细胞周期素依赖性蛋白激酶抑制物(CKI) |
| 收稿时间： | 2005-07-24 |
| 修稿时间： | 2005-07-242005-08-30 |
Effects of NGX6 Gene on Cell Cycle in Colon Cancer Cell Line HT-29 |
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| WANG Xiao-Yan,SHEN Shou-Rong,LIU Fen,LI Xiao-Ling and FAN Song-Qing.Effects of NGX6 Gene on Cell Cycle in Colon Cancer Cell Line HT-29[J].Progress In Biochemistry and Biophysics,2006,33(1):45-50. |
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| Authors: | WANG Xiao-Yan SHEN Shou-Rong LIU Fen LI Xiao-Ling and FAN Song-Qing |
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| Institution: | Department of Gastroenterology, Xiangya Third Hospital, Central South University, Changsha 410013, China;Department of Gastroenterology, Xiangya Third Hospital, Central South University, Changsha 410013, China;Department of Gastroenterology, Xiangya Third Hospital, Central South University, Changsha 410013, China;Cancer Research Institute, Xiangya Medical School of Center South University, Changsha 410013, China;Cancer Research Institute, Xiangya Medical School of Center South University, Changsha 410013, China |
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| Abstract: | NGX6, a candidate of tumor suppressor genes, can inhibit the proliferation of colon cancer in vivo andin vitro. The uncontrolled proliferation of tumor cell closely related with the dysfunction of the cell cycle. Flowcytometry and Western blot were used to explore effects of NGX6 on cell cycle progression and the expression ofcyclins, CKI(p27,p16) in colon cancer cell line HT-29. Flow cytometry (FCM) showed that the overexpression ofNGX6 increased the proportion of HT-29 cells at G0/G1 phase and decreased that at S, G2 and M phase. Westernblot and FCM indicated that NGX6 could down-regulate the expression of cyclin E, cyclin D1 and increase the p27expression,while NGX6 has no significant effect on expression of cyclin A and cyclin B. The expression of p16was not detected in all groups. These results demonstrated that NGX6 arrested cell cycle progression at G0/G1phase by down-regulating the expression of cyclin E, cyclin D1 and up-regulating that of p27 to inhibit theproliferation of HT-29 cells. |
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| Keywords: | NGX6 gene HT-29 cell cell cycle cyclins cyclin-dependent kinase inhibitor (CKI) |
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