一种免受乙醇干扰的GOD的酶电极

将酶电极应用于发酵糖的测定时。与糖共存的乙醇常常会影响测糖的准确性，通过对葡萄糖氧化酶(GOD)电极的研究，探讨了乙醇对测糖酶电极测定影响，进而研制出抗干扰的GOD酶电极，若是GOD电极的酶膜通过夹心法制备．在乙醇含量为0．1％(V／V)时·即产生显著的影响，使测定结果偏大4．3%，且乙醇的影响随浓度的升高而增大，若用尼龙网固定GOD膜，GOD电极在测定20mmol／L和5mm01／L左右的葡萄糖溶液时，含量高达9％的乙醇仍未对测定产生显著的影响．表现出良好的不受乙醇干扰的特性，并且,该尼龙网GOD电极具有良好的重复性、稳定的响应活性及较长的保存期．

A GOD Electrode Free from the Influence of Alcohol

The influence of alcohol on GOD electrode was investigated. When enzyme electrode was made by Sandwich method, 0. 1% (V/V) alcohol had an obvious influence on the response of the electrode, the result deviated by +4. 3%. The influence increased with the increase of alcohol content of'samples. This influence can be avoided by using a nylon mesh GOD electrode, which was successfully used for determination of glucose in range of 5-20mmol/L with alcohol content up to 9% (V/V). Such an electrode showed a good reproducibility, stable response activity and long storage life (>30 days).